Phospho-eIF4E (S209) Recombinant Rabbit Monoclonal Antibody [SU0396]
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.In addition to its role in translation initiation, also acts as a regulator of translation and stability in the cytoplasm.Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression: in the complex, EIF4E mediates the binding to the mRNA cap.Component of a multiprotein complex that sequesters and represses translation of proneurogenic factors during neurogenesis.In P-bodies, component of a complex that mediates the storage of translationally inactive mRNAs in the cytoplasm and prevents their degradation.May play an important role in spermatogenesis through translational regulation of stage-specific mRNAs during germ cell development.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
eIF-4E, eIF4E, EIF4E
Swiss Prot
P06730 Human, P63073 Mouse, P63074 Rat
Cellular Locus
Cytoplasm, Nucleus.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser209 of human eIF4E.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, IHC-P, IP
Positive Control
Concentration
1 mg/mL
Dilution
WB: 1:500-1:2,000;IF-Cell: 1:50-1:200;IHC-P: 1:50-1:200;IP: Use at an assay dependent concentration.
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 25 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
Frequently Asked Questions
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