CD34 Recombinant Rabbit Monoclonal Antibody [SI16-01]
CD34 is a heavily glycosylated, transmembrane glycoprotein that is expressed on the surface of lymphohematopoietic stem and progenitor cells, small-vessel endothelial cells, embryonic fibroblasts and some cells in fetal and adult nervous tissue. CD34 antigen expression is highest in the most primitive stem cells and is gradually lost as lineage committed progenitors differentiate. The CD34 antigen is also present on capillary endothelial cells and on bone marrow stromal cells. The CD34 cytoplasmic domain has an intracellular domain that contains consensus sites for activated protein kinase C (PKC) phosphorylation as well as serine, threonine and tyrosine phosphorylation consensus sites.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
CD34
Swiss Prot
P28906 Human, Q64314 Mouse
Cellular Locus
Membrane.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthetic peptide within Human CD34 aa 336-385 / 385.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, IF-Tissue, IHC-P, IP, mIHC, FC
Positive Control
Concentration
1 mg/mL
Dilution
WB: 1:2,000 ;IF-Cell: 1:100 ;IF-Tissue: 1:500-1:1,000 ;IHC-P: 1:400-1:10,000 ;IP: 1-2μg/sample ;mIHC: 1:1,000-1:2,000 ;FC: 1:1,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 41 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
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