CD20 Recombinant Rabbit Monoclonal Antibody [SY12-01]
CD20 is a leukocyte surface antigen consisting of four transmembrane regions and cytoplasmic N- and C-termini. The cytoplasmic domain of CD20 contains multiple phosphorylation sites, leading to additional isoforms. CD20 is expressed primarily on B cells but has also been detected on both normal and neoplastic T cells. CD20 functions as a calcium-permeable cation channel, and it is known to accelerate the G0 to G1 progression induced by IGF-1. CD20 is activated by the IGF-1 receptor via the alpha subunits of the heterotrimeric G proteins. Activation of CD20 significantly increases DNA synthesis and is thought to involve basic helix-loop-helix leucine zipper transcription factors.
Product Specifications
Product Name Alternative
Abbreviation
MS4A1
Swiss Prot
P11836 Human
Cellular Locus
Cell membrane.
Host
Rabbit
Species Reactivity
Human,Monkey
Immunogen
Synthetic peptide within Human CD20 aa 248-297 / 297.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IHC-P, IF-Tissue, IF-Cell, FC, IP
Positive Control
Raji cell lysate, Ramos cell lysate, Daudi cell lysate, human tonsil tissue, human spleen tissue, B-cell lymphoma tissue, Raji.
Concentration
1 mg/mL
Dilution
WB: 1:1,000-1:5,000 ;IHC-P: 1:200 ;IF-Tissue: 1:50 ;IF-Cell: 1:100 ;FC: 1:1,000 ;IP: 1-2μg/sample
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 33 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
CAS Number
9000-83-3
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