Histone H3 (mono methyl K36) Recombinant Rabbit Monoclonal Antibody [SR04-20]
Selected Variant
ET1602-48
Size: 100 µL
In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. Histone H3, the core protein of the nucleosome, becomes phosphorylated at the end of prophase. The two major sites of phosphorylation are the mitosis-specific site Ser10, and Ser28, both of which are extensively phosphorylated in DNA-bound forms of histone H3 and in nucleosomal histone H3. The nucleosome structure of histone H3 promotes N-terminal phosphorylation in vitro.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
H3f3a H3K36me1
Swiss Prot
P68431 Human, P84243 Human, Q16695 Human, Q6NXT2 Human, Q71DI3 Human, P84244 Mouse
Cellular Locus
Nucleus, Chromosome.
Host
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Synthetic peptide within N-terminal human Histone H3.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, IF-Tissue, ChIP
Positive Control
Hela cell lysate, NIH/3T3 cell lysate, MCF-7.
Concentration
1 mg/mL
Dilution
WB: 1:1,000-1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 15 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
Price
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