Firefly Luciferase Recombinant Rabbit Monoclonal Antibody [SR03-86]
Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
ec 1.13.12.7 antibody Firefly antibody Luciferase antibody Luciferin 4 monooxygenase antibody
Abbreviation
Luciferase
Swiss Prot
P08659 Firefly
Cellular Locus
Peroxisome.
Host
Rabbit
Species Reactivity
Firefly
Immunogen
Recombinant protein within Firefly Luciferase aa 132-243 / 550.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, IF-Tissue
Positive Control
Luciferase recombinant protein.
Concentration
1 mg/mL
Dilution
WB: 1:500-1:2,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 61 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
Frequently Asked Questions
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