ASK1 Rabbit Polyclonal Antibody
Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also designated MAP kinase kinase kinases, MKKKs, MAP3Ks or MEKKs) phosphorylate and thereby activate the MEKs (also called MAP kinase kinases or MKKs), including ERK, JNK and p38. These activated MEKs in turn phosphorylate and activate the MAP kinases. The MEK kinases include Raf-1, Raf-B, Mos, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4, ASK 1 (MEK kinase-5) and MAP3K6 (MEK kinase-6). MEK kinase-1 has been shown to phosphorylate MEK-1 via a Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK pathway. ASK1 activates both MEK-4 and MEK-3/MEK-6 pathways.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Abbreviation
ASK-1, MEK kinase 5, MEKK 5, MAP3K5, ASK1, MAPKKK5, MEKK5
Swiss Prot
Q99683 Human, O35099 Mouse
Cellular Locus
Cytoplasm, Endoplasmic reticulum.
Host
Rabbit
Species Reactivity
Human,Mouse
Immunogen
Synthetic peptide of C-terminal human ASK1.
Isotype
IgG
Conjugation
Non-conjugated
Type
Rabbit polyclonal Antibody
Applications
WB, IF-Cell, IHC-P
Positive Control
Human skeletal muscle tissue lysate, human colon cancer tissue, mouse testis tissue, PANC-1.
Concentration
1 mg/mL
Dilution
WB: 1:500-1:1,000;IF-Cell: 1:50-1:200;IHC-P: 1:50-1:200
Purity
Immunogen affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 155 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
No
Frequently Asked Questions
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