BubR1 Rabbit Polyclonal Antibody
Human cells contain two related protein kinases, BUB1 and BUBR1, that appear to have evolved from a single ancestral BUB1 gene. Both kinases are concentrated near the surface of the kinetochore where they monitor kinetochore-microtubule interactions. BUB1 and BUBR1 bind to kinetochores and are postulated to be components of the mitotic checkpoint, which monitors kinetochore activities to determine if chromosomes have achieved alignment at the spindle equator. BUBR1 is essential for normal mitotic progression as it prevents cells from prematurely entering anaphase. BUB3 is a conserved component of the mitotic spindle assembly complex and is also involved with the essential spindle checkpoint pathway that operates during early embryogenesis.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Abbreviation
hBUBR1, BUB1B, BUBR1, MAD3L, SSK1
Swiss Prot
O60566 Human
Cellular Locus
Nucleus, Cytoplasm, Cytoskeleton.
Host
Rabbit
Species Reactivity
Human
Immunogen
Recombinant protein within human BubR1 aa 310-620.
Isotype
IgG
Conjugation
Non-conjugated
Type
Rabbit polyclonal Antibody
Applications
WB, IF-Cell, IHC-P, FC
Positive Control
SH-SY-5Y cell lysate, Hela, SH-SY-5Y, SiHa, human spleen tissue, HL-60.
Concentration
1 mg/mL
Dilution
WB: 1:5,000-1:10,000;IF-Cell: 1:50-1:200;IHC-P: 1:50-1:200;FC: 1:50-1:100
Purity
Immunogen affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 120 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
No
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