Ki67 Rabbit Polyclonal Antibody
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Abbreviation
Antigen KI-67, Antigen Ki67, MKI67
Swiss Prot
P46013 Human
Cellular Locus
Nucleus.
Host
Rabbit
Species Reactivity
Human
Immunogen
Synthetic peptide within human Ki67 aa 1009-1094.
Isotype
IgG
Conjugation
Non-conjugated
Type
Rabbit polyclonal Antibody
Applications
WB, IF-Cell, IHC-P, FC
Positive Control
HepG2, A431, A549, LOVO, human tonsil tissue, human lung cancer tissue, human colon cancer tissue, human stomach cancer tissue.
Concentration
1 mg/mL
Dilution
WB: 1:500-1,000;IF-Cell: 1:200-1:1,000;IHC-P: 1:100-1:500;FC: 1:50-1:200
Purity
Immunogen affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 359 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
No
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