CRISPR-Cas9 Mouse Monoclonal Antibody [8-A6-6]
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Type II system is currently the most commonly used RNA-Guided Endonuclease technology for genome engineering. There are two distinct components to this system: (1) a guide RNA and (2) an endonuclease, in this case the CRISPR associated (Cas) nuclease, Cas9. The gRNA/Cas9 complex is recruited to the target sequence by the base-pairing between the gRNA sequence and the complement to the target sequence in the genomic DNA. For successful binding of Cas9, the genomic target sequence must also contain the correct Protospacer Adjacent Motiff (PAM) sequence immediately following the target sequence.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Cas9 antibody CRISPR-associated endonuclease Cas9/Csn1 antibody CRISPR-Cas9/Csn1 antibody csn1 antibody SpyCas9 antibody
Swiss Prot
Q99ZW2 Streptococcus Pyogenes
Host
Mouse
Species Reactivity
Staphylococcus aureus
Immunogen
Recombinant protein within Streptococcus Pyogenes CRISPR-Cas9 aa1-200.
Isotype
IgM
Conjugation
Non-conjugated
Type
Mouse monoclonal Antibody
Applications
WB, IF-Cell
Positive Control
Human 293T cells transfected with CRISPR-Cas9 lysate.
Concentration
2 mg/mL
Dilution
WB: 1:5,000 ;IF-Cell: 1:200-1:1,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
No
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