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T7 tag Mouse Monoclonal Antibody [4-C2]

The T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems. This antibody recognizes the T7-tag fused to either the amino- or carboxy-terminus of targeted proteins.

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

10 antibody Major capsid protein 10A antibody Major capsid protein antibody Minor capsid protein 10B antibody T7 epitope tag antibody

Host

Mouse

Species Reactivity

Universal

Immunogen

Synthetic peptide CMASMTGGQQMG.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, ELISA, IP, IF-Cell

Positive Control

T7-tagged recombinant protein.

Concentration

2 mg/mL

Dilution

WB: 1:1,000 ;ELISA: Use at an assay dependent concentration. ;IF-Cell: Use at an assay dependent concentration. ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No

Frequently Asked Questions

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