GADD34 Mouse Monoclonal Antibody [A4D7]
Protein phosphatase 1 regulatory subunit 15A also known as growth arrest and DNA damage-inducible protein GADD34 is a protein that in humans is encoded by the PPP1R15A gene. The Gadd34/MyD116 gene was originally discovered as a member in a set of gadd and MyD mammalian genes encoding acidic proteins that synergistically suppress cell growth. Later on it has been characterized as a gene playing a role in ER stress-induced cell death, being a target of ATF4 that plays a role in ER-mediated cell death via promoting protein dephosphorylation of eIF2α and reversing translational inhibition. This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Abbreviation
PPP1R15A
Swiss Prot
O75807 Human
Cellular Locus
Endoplasmic reticulum membrane, Mitochondrion outer membrane.
Host
Mouse
Species Reactivity
Human
Immunogen
Synthetic peptide within Human GADD34 aa 1-50 / 674.
Isotype
IgG1
Conjugation
Non-conjugated
Type
Mouse monoclonal Antibody
Applications
WB, IHC-P
Positive Control
A549 cell lysate, HepG2 cell lysate, K562 cell lysate, human prostate carcinoma tissue.
Concentration
2 mg/mL
Dilution
WB: 1:500-1:1,000 ;IHC-P: 1:50-1:200
Purity
Protein G affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 73 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
No
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