APE1 Mouse Monoclonal Antibody [12H4]
The role of transcription factors in the regulation of gene expression is well established. Although the activity of these factors can be regulated by phosphorylation, evidence has indicated regulation of DNA binding mediated by changes in reduction-oxidation (redox) status. Mutational analysis has identified a single conserved cysteine residue mapping within the DNA binding domains of Fos and Jun. Chemical oxidation or modification of this cysteine residue inhibits the DNA binding activity of Fos and Jun. A similar mode of regulation has been recently proposed for other nuclear transcription factors. Oxidation is reversible by these compounds or by a cellular redox/DNA repair protein identified originally as Ref-1 (redox factor 1). Ref-1 is identical to a previously characterized DNA repair enzyme designated HAP1, APE or APEX.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Abbreviation
APEN, AP endonuclease 1, APE-1, APEX1, APE, APE1, APEX, APX, HAP1, REF1
Swiss Prot
P27695 Human, P28352 Mouse
Cellular Locus
Nucleus. Endoplasmic reticulum.
Host
Mouse
Species Reactivity
Human,Mouse
Immunogen
Recombinant protein within Human APE1 aa 20-318/318.
Isotype
IgG1
Conjugation
Non-conjugated
Type
Mouse monoclonal Antibody
Applications
WB, IHC-P, FC
Positive Control
HL-60, human liver tissue, human prostate cancer tissue, human kidney tissue, mouse colon tissue, SiHa.
Concentration
2 mg/mL
Dilution
WB:1:1,000-1:5,000 ;IHC-P:1:50-1:200 ;FC:1:50-1:100
Purity
Immunogen affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 36 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
No
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