HLA Class 1 ABC Mouse Monoclonal Antibody [11C3]
HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.
Product Specifications
Product Name Alternative
Abbreviation
HLA-A, HLAA, HLA-B, HLAB, HLA-C, HLAC
Swiss Prot
P04439 Human, P01889 Human, P10321 Human
Cellular Locus
Cell Membrane, Endoplasmic reticulum membrane.
Host
Mouse
Species Reactivity
Human
Immunogen
Recombinant protein within Human HLA Class 1 ABC aa 1-362 / 362.
Isotype
IgG1
Conjugation
Non-conjugated
Type
Mouse monoclonal Antibody
Applications
WB, IF-Cell, IHC-P
Positive Control
CAL-62 cell lysate, HeLa cell lysate, HepG2 cell lysate, human liver tissue, 293T.
Concentration
2 mg/mL
Dilution
WB:1:500-1:2000 ;IF-Cell:1:50-1:200 ;IHC-P: 1:5,000
Purity
Immunogen affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 40 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
No
CAS Number
9007-83-4
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