BHMT Mouse Monoclonal Antibody [9E1]
Betaine-homocysteine methyltransferase (BHMT) is a zinc-dependent cytosolic protein that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. BHMT is highly expressed in rat liver, and its expression is regulated by dietary methionine and choline. In humans, S-adenosylmethionine (SAM) down-regulates BHMT expression by inducing NFκB, which acts as a repressor for the BHMT gene. Lowered BHMT levels can lead to ER (endoplasmic reticulum) stress. Mutations in the gene encoding for BHMT may lead to hyperhomocysteinemia, a medical condition characterized by abnormally large amounts of homocysteine in the blood which may be a risk factor for cardiovascular and cerebrovascular diseases.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Abbreviation
BHMT
Swiss Prot
Q93088 Human, 329582 Mouse, O09171 Rat
Cellular Locus
Cytoplasm.
Host
Mouse
Species Reactivity
Human,Mouse,Rat
Immunogen
Recombinant protein with Human BHMT aa 250-406 / 406.
Isotype
IgG1
Conjugation
Non-conjugated
Type
Mouse monoclonal Antibody
Applications
WB, IHC-P, IF-Cell, FC
Positive Control
Human liver tissue lysate, human kidney tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue, SiHa, HepG2.
Concentration
2 mg/mL
Dilution
WB: 1:1,000-1:20,000 ;IF-Cell: 1:50 ;IHC-P: 1:2,000 ;FC: 1:50-1:100
Purity
Protein G affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 45 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
No
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