BAX Mouse Monoclonal Antibody [3-D2]
BAX is a member of the Bcl-2 gene family. Apoptosis regulator BAX promotes apoptosis by binding to and antagonizing the Bcl-2 protein. In healthy mammalian cells, the majority of BAX is found in the cytosol, but upon initiation of apoptotic signaling, Bax undergoes a conformational shift. Upon induction of apoptosis, BAX becomes organelle membrane-associated, and in particular, mitochondrial membrane associated. The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis. The p53 protein is a transcription factor that, when activated as part of the cell's response to stress, regulates many downstream target genes, including BAX.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Abbreviation
Bcl2-L-4
Swiss Prot
Q07812 Human, Q07813 Mouse
Cellular Locus
Mitochondrion membrane, cytoplasm
Host
Mouse
Species Reactivity
Human,Mouse
Immunogen
This antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to N-terminal BAX.
Isotype
IgA
Conjugation
Non-conjugated
Type
Mouse monoclonal Antibody
Applications
WB, IF-Cell, IHC-P, FC
Positive Control
Daudi cell lysate, Raji cell lysate, HepG2, Hela.
Concentration
2 mg/mL
Dilution
WB: 1:500;IF-Cell: 1:200;IHC-P: 1:200;FC: 1:50
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 21 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
No
Frequently Asked Questions
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