Oct4 Mouse Monoclonal Antibody [A3-C11]
Oct-4 (POU5F1) is transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). It forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Oct-4 is critical for early embryogenesis and for embryonic stem cell pluripotency. It is expressed in developing brain and highest levels also found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Abbreviation
POU5F1, OCT3, OCT4, OTF3
Swiss Prot
Q01860 Human
Cellular Locus
Cytoplasm, Nucleus.
Host
Mouse
Species Reactivity
Human,Mouse
Immunogen
Recombinant protein within full length human Oct4 protein.
Isotype
IgG1
Conjugation
Non-conjugated
Type
Mouse monoclonal Antibody
Applications
WB, IF-Cell, FC
Positive Control
NCCIT cell lysate, D3 cell lysate, NCCIT.
Concentration
2 mg/mL
Dilution
WB: 1:1,000-1:5,000 ;IF-Cell: 1:200 ;FC: 1:1000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 39 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
No
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