EGFR Rabbit Polyclonal Antibody
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascad.
Product Specifications
Product Name Alternative
Abbreviation
Egfr
Swiss Prot
P00533 Human, Q01279 Mouse,
Cellular Locus
Plasma membrane, Nucleus, Endosome, Endoplasmic reticulum.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthetic peptide within mouse EGFR aa 11-60 / 1,210.
Isotype
IgG
Conjugation
Non-conjugated
Type
Rabbit polyclonal Antibody
Applications
WB, IF-Cell, IHC-P
Positive Control
A431 cell lysates, A172, rat stomach tissue, human stomach cancer tissue.
Concentration
1 mg/mL
Dilution
WB: 1:500-1:1,000;IF-Cell: 1:50-1:200;IHC-P: 1:50-1:200
Purity
Immunogen affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 134 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
No
CAS Number
9007-83-4
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