Chicken anti Human Osteopontin c (no preservative)

A chicken was immunized with a peptide representing the splice junction of osteopontin-c (Ac-SEEKQNAVSC-COOH). After multiple immunizations, eggs were collected and IgY was purified from the egg yolk. The antibody was affinity purified on an osteopontin-c peptide column. No sodium azide was added.

This antibody has been tested in ELISA and a sµggested starting dilution for ELISA analysis is 2 µg/mL. Optimal working dilutions should be determined for your particular assay conditions. For Western blotting, recommended concentration 1 µg/mL.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

1. He B, Mirza M, Weber GF., 2006. An osteopontin splice variant induces anchorage independence in Human breast cancer cells. Oncogene 25:2192–2202._x000D_ 2. Mirza M, Shaughnessy E, Hurley JK, Vanpatten KA, Pestano GA, He B, Weber GF., 2008. Osteopontin-c is a selective marker of breast cancer. Int J Cancer: 122:2646._x000D_ 3. Sullivan J, Blair L, Alnajar A, Aziz T, Ng CY, Chipitsyna G, Gong Q, Witkiewicz A, Weber GF, Denhardt DT, Yeo CJ, Arafat HA., 2009. Expression of a prometastatic splice variant of osteopontin, OPNC, in Human pancreatic ductal adenocarcinoma. Surgery 146:232-40._x000D_ 4. Tilli, TM, Thuler LC, Matos, AR, Coutinho-Camillo, CM, Soares, FA, da Silva, EA, Neves, AF, Goulart, LR and ER Gimba. 2011.Expression analysis of osteopontin mRNA splice variants in prostate cancer and benign prostatic hyperplasia. Exp. Mol. Pathol. 92:13-19._x000D_ 5. Tilli, TM, Mello, KD, Ferreira, LB, Matos, AR, Accioly, MTS, Faria, PAS., Bellahcène, A, Castronovo, V and ER Gimba. 2012. Both osteopontin-c and osteopontin-b splicing isoforms exert pro-tumorigenic roles in prostate cancer cells. Prostate 72:1688-1699._x000D_ 6. Zhang, MX, Xu YJ, Zhu MC and F. Yan. 2013. Overexpressed Osteopontin-c as a Potential Biomarker for Esophageal Squamous Cell Carcinoma. Asian Pac J Cancer Prev 14: 7315-9 _x000D_ _x000D_ 7. T. Martins Tilli, L. Bueon Ferreira and E.R. Pereira Gimba. 2015. Osteopontin-c mediates the upregulation of androgen responsive genes in LNCaP cells through P13K/Akt and androgen receptor signaling. Oncol. Lett. 9: 1845-50. _x000D_ 8. Zduniak K., Ziolkowski, P. Ahlin C., Agrawal, A., Blomqvist, C., Fjallskog, M.L., and G.F. Weber. 2015. Nuclear Osteopontin-c is a prognostic breast cancer marker. Br. J. Cancer 112:729-38_x000D_ 9. Zduniak, K, Agrawal, A, Agriwal, S, Hossain, MM, Ziolkowski, P and GF Weber, 2016. Osteopontin splice variants are differential predictors of breast cancer treatment responses. BMC Cancer 16:441.