Anti-DFF40/CAD (IN) Antibody

Rabbit Polyclonal Antibody specific to DFF40 (IN)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 3.-.-.-, Caspase-activated deoxyribonuclease, CAD, Caspase-activated DNase, DNA fragmentation factor 40 kDa subunit, DFF-40

Gene Name

Dffb

Gene ID

13368

NCBI Gene ID

UniProt

O54788

Accession Number

NP_031885

Cellular Locus

Cytoplasm. Nucleus.

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide corresponding to aa 203-218 of human DFF40 (accession no. NP_004393).

Target Antigen

DNA fragmentation factor subunit beta

Target

DFF40/CAD (IN)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, ICC

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.

Additionnal Information

Immunoblotting: use at 1-2ug/mL. Immunocytochemistry: use at 10ug/mL. Positive control: Whole cell lysate of Jurkat cells. These are recommended concentrations. Enduser should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human, mouse, and rat DFF40/CAD (40kDa).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Cell death signals are transduced by death domain-containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse Dnase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxy- ribonuclease). The human homologue of mouse CAD was more recently identified and termed CPAN, DFF40, and human CAD. DFF45/ICAD is the inhibitory protein of DFF40/CAD with which it forms complexes. Upon cleavage by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.