FGF-2 (basic) heat stable, 154 aa

FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic exerts proliferative activity towards a wide range of cell types and consequently is used as a vital component in numerous cell culture media formulations, including stem cell media. FGF-basic loses its activity over time in solution at physiological temperatures and needs to be resupplemented periodically to maintain its performance. As a result of selected amino acid substitutions, Heat Stable FGF-basic retains full biological activity and also maintains full activity over extended periods of time in solution at 37°C. These properties make Heat Stable FGF-basic a superior choice for use in cell culture media applications. Heat Stable Recombinant Human FGF-basic is a 17.2 kDa protein consisting of 154 amino acids.

Product Specifications

Synonyms

Fibroblast Growth Factor-basic, FGF-2, HBGF-2, Prostatropin

NCBI Gene ID

2247

UniProt

P09038

Accession Number

NP_001997.5

Accession Number mRNA

NM_002006.4

Chromosomal Location

4q26

Reactivity

Human

Cross Reactivity

Human

Sequence

AAGSITTLPA LPEDGGSGAF PPGHFKDPKL LYCKNGGFFL RIHPDGRVDG TRDKSDPFIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL YAIKNVTDEC FFFERLEENN YNTYRSRKYP SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS

Assay Protocol

Centrifuge vial prior to opening. Reconstitute in 5mM Tris, pH 7.6, to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Endotoxin

< 0.1 ng/µg of protein (< 1EU/µg)

Purity

≥ 95% by SDS-PAGE gel and HPLC analyses.

Bioactivity

Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is 0.05-0.25 ng/ml.

Length

154

Form

Lyophilized

Buffer

5mM Tris, pH 7.6 + 150mM NaCl

Reconstitution

5mM Tris, pH 7.6

Molecular Weight

17.2 kDa

Storage Conditions

The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 12 months at -20°C to -80°C.