Anti-K+/Cl- Cotransporter (KCC2) Antibody

Mouse Monoclonal Antibody specific to K+/Cl-Cotransporter (KCC2)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Electroneutral potassium-chloride cotransporter 2, Furosemide-sensitive K-Cl cotransporter, K-Cl cotransporter 2, rKCC2, Neuronal K-Cl cotransporter

Gene Name

Slc12a5

NCBI Gene ID

UniProt

Q63633

Accession Number

NP_599190

Cellular Locus

Membrane; Multi-pass membrane protein.

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein consisting of aa 932-1043 of rat KCC2 (accession no. NP_599190).

Target Antigen

Solute carrier family 12 member 5

Target

K+/Cl- Cotransporter (KCC2)

Clonality

Monoclonal

Isotype

IgG2a

Type

Antibody

Applications

WB, IHC, ICC/IF, IP

Field of Research

Ion Channels

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl(-) homeostasis. As major extruder of intracellular chloride, it establishes the low neuronal Cl(-) levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition (PubMed:9930699). Involved in the regulation of dendritic spine formation and maturation (PubMed:22345354). {PubMed:22345354, PubMed:9930699}.

Additionnal Information

Immunoblotting: use at 3ug/mL. A band of ~140kDa is detected. Immunohistochemistry: use at 1-5ug/mL. Positive control: Adult rat brain These are recommended concentrations; Enduser should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse and rat KCC2.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

KCC2 is a member of the cation-chloride cotransporter gene family. KCCs lower intracellular chloride concentrations below the electrochemical equilibrium potential. Depending on the concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. KCC2 is expressed at high levels in neurons throughout the nervous system and is localized at inhibitory synapses of the spinal cord. Studies in mice have shown that KCC2 reduces GABA's inhibitory signaling resulting in motor defects, epilepsy, and anxiety-like behavior.