Human Integrin alpha D beta 2 (ITGAD&ITGB2) Heterodimer Protein, His Tag&Tag Free

Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. May play a role in the atherosclerotic process such as clearing lipoproteins from plaques and in phagocytosis of blood-borne pathogens, particulate matter, and senescent erythrocytes from the blood. Contributes to natural killer cell cytotoxicity. Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (PubMed:18587400) . Alpha D beta 2, selectively binds ICAM-3 and does not appear to bind ICAM-1. In addition, a soluble form of alpha D beta 2 binds VCAM-1 with greater efficiency relative to ICAM-3. The I domain of alpha D contains a binding site for VCAM-1 since recombinant alpha D I domain binds specifically to VCAM-1.

Product Specifications

Background

Specifications

Human Integrin alpha D beta 2 (ITGAD&ITGB2) Heterodimer Protein, produced by co-expression of ITGAD and ITGB2, has a calculated MW of 124.9 kDa (ITGAD) and 80.2 kDa (ITGB2) . Subunit ITGAD is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The protein migrates as 150 kDa (ITGAD) and 90-100 kDa (ITGB2) under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q13349-1 (ITGAD) & P05107-1 (ITGB2)

Expression Region

Phe 18 - Asn 1099 (ITGAD) & Gln 23 - Asn 700 (ITGB2)

Host

CHO

Target

Integrin alpha D beta 2

Conjugation

Unconjugated

Tag

C-10xHis | Native

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human ITGAD&ITGB2 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT2-H53W6) at 1 μg/mL (100 μL/well) can bind Human Integrin beta 2/CD18 Antibody with a linear range of 0.3-5 ng/mL (QC tested).