Human IDH1 (R132C) Protein, His Tag (MALS verified)
The IDH enzymes catalyze the oxidative decarboxylation of isocitrate to alpha-ketoglutarate (α-KG), producing nicotinamide adenine dinucleotide phosphate (NADPH) in the process via the citric acid cycle. Eukaryotic cells express two distinct classes of IDHs that utilize either NAD or NADP as their cofactors and serve diverse biological functions. NAD-dependent IDH is localized to the mitochondrial matrix and is well known for its central role for energy production in the Krebs cycle. NADP-dependent IDHs are primarily located either in mitochondria or cytoplasm . Each NADP-dependent isozyme is a homodimer. Mutations of Arg132 of human IDH1 result in a reduced ability of the enzyme to convert isocitrate to alpha ‑ketoglutarate, but the enzyme acquires the ability to generate 2-hydroxyglutarate (2HG) from alpha‑ketoglutarate, 2-HG is elevated in several tumor types, including a subset of AMLs.
Product Specifications
Background
Specifications
Accession Number
O75874-1 (R132C)
Expression Region
Met 1 - Leu 414
Host
E. coli
Target
IDH1
Conjugation
Unconjugated
Tag
C-10xHis
Source
Human
Stability
The product MUST be stored at -70°C or lower upon receipt; -70°C for 3 months under sterile conditions.
Endotoxin
1.0 EU per μg
Purity
95%
Format
Liquid
Buffer
50 mM Tris, 150 mM NaCl, pH7.5
Additives
Glycerol
Molecular Weight
48.5 kDa
Additionnal Information
Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments
Shipping Conditions
Dry ice
Storage Conditions
-70°C
Package Size
500ug*1
Host or Source
E. coli
Species
Human
Protein ID
O75874-1 (R132C)
Preservative
Glycerol
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