Fluorescein-12-dATP

Fluorescein-12-dATP is a fluorescent dye acting as the detection probe. Fluorescein-12-dATP can be used to label the thrombin-binding aptamers by catalytic polymerization of TdT (terminal deoxynucleotidyl transferase) [1]. Fluorescein-12-dATP is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

Product Specifications

UNSPSC

12171500

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/fluorescein-12-datp.html

Solubility

10 mM in DMSO

Smiles

O=C(NCC#CC1=CN(C2=C1C(N)=NC=N2)[C@@]3(O[C@@H]([C@H](C3)O)COP(OP(OP(O)(O)=O)(O)=O)(O)=O)[H])CCCCCNC(C4=CC=C(C(C(O)=O)=C4)C5=C(C(OC6=C5C=CC(O)=C6)=C7)C=CC7=O)=O

Molecular Formula

C41H41N6O19P3

Molecular Weight

1014.71

References & Citations

[1]Wen D, et al. Highly sensitive fluorometric determination of thrombin by on-chip signal amplification initiated by terminal deoxynucleotidyl transferase. Mikrochim Acta. 2018 Jul 19;185 (8) :380.