Recombinant Mouse CCL12 Protein (Trx Tag)

Product Specifications

Background

Ccl12 prevented initiation of the reparative response by prolonging inflammation and inhibiting fibroblast conversion to myofibroblasts, resulting in diminished scar formation. Macrophage secretion of Ccl12 directly impaired fibronectin and collagen deposition and indirectly stimulated collagen degradation through upregulation of matrix metalloproteinase-2. In post-MI patients, circulating LPS levels strongly associated with the Ccl12 homologue monocyte chemotactic protein 1 (MCP-1) . Both MCP-1 and MCP-5 are HIF-1 target genes and that HIF-1alpha is involved in transcriptional induction of these two chemokines in astrocytes by hypoxia.

Abbreviation

CCL12

Synonyms

Ccl; Mcp; Scya; CCL12/MCP; C-C motif chemokine; Small-inducible cytokine A; Monocyte chemoattractant protein; CCL12/MCP-5; CCL12; Mcp5; Scya12; C-C motif chemokine 12; MCP-1-related chemokine; Monocyte chemoattractant protein 5; Monocyte chemotactic protein 5 (MCP-5) ; Small-inducible cytokine A12

UniProt

Q62401

Accession Number

Q62401

Expression System

E.coli

Tag

N-Trx

Sequence

Gly23-Gly104

Field of Research

Immunology; Cell Biology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.