Human Anti-SARS-CoV-2 (BF.7) Antibody IgA2 Titer Serologic Assay Kit (Spike RBD)

It's been reported that coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

1. Unopened kit should be stored at 2°C-8°C upon receiving. 2. Find the expiration date on the outside packaging and do not use reagents past their expiration date. 3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

This assay kit is used to measure the levels of Anti-SARS-CoV-2 antibody IgA2 by employing an indirect ELISA format. The microplate in the kit has been pre-coated with SARS-CoV-2 Spike RBD(BF.7). First add the diluted samples to the plate and wash the wells after incubation. Afterwards add HRP-Anti-Human IgA2 to the plate and wash the wells after incubation. Lastly the substrate is loaded into the wells and color develops in proportion to the amount of antibody. The reaction is stopped by the addition of a stop solution and the intensity of the color can be measured at 450 nm. The OD Value reflects the amount of antibody bound.