Anti-CD19 (FMC63) CAR Immunogenicity ELISA Kit

FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.Anti-fmc63 scFv antibody can specifically bind to the antigen recognition epitope of fmc63 scFv on anti-CD19 car, and it shows high specificity and sensitivity,it's used to detect the expression of fmc63 scFv derived car.

This kit is developed for detection of Anti-CD19 (FMC63) antibodies in CAR Immunogenicity assay test. Detection and monitoring the level of ADA is important to fully understand the potential immune responses.

1. Store the unopened kit at 2-8°C upon receipt. 2. Locate the expiration date on the outer packaging and do not use reagents beyond their expiration date. 3. The opened kit should be stored according to the storage conditions listed in the Components Table. The shelf life of the opened kit is 30 days from the date of opening.

This assay kit employs a bridging-ELISA format, providing a rapid detection of FMC63 ADA. The kit consists High-bind Plate and Mouse FMC63 scFv and FMC63 ADA Standard and Biotin-Mouse FMC63 scFv and Streptavidin-HRP and buffers.