Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2)

Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) is a monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply.

Product Specifications

Background

CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.

CAS Number

9007-83-4

Host

HEK293

Origin Species

Mouse

Target

CD3

Conjugation

Unconjugated

Tag

Mouse IgG1 | Mouse lambda

Applications

ELISA (Evaluation of levels of CD3 epsilon protein) . Flow Cytometry (Evaluation of an epitope within the extracellular domain of CD3 epsilon protein) .

Stability

-20°C to -70°C for 24 months in lyophilized state; -70°C for 12 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles.

Bioactivity

Immobilized Human CD3 epsilon, His Tag (Cat. No. CDE-H5223) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) (Cat. No. CDE-M531) with a linear range of 0.4-6 ng/mL (QC tested).