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Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 (AM223) (MALS verified)

Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 is a chimeric monoclonal antibody recombinantly expressed from CHO cells, which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain. The mouse monoclonal antibody was obtained from a mouse immunized with recombinant SARS-CoV-2 Nucleocapsid Protein. This chimeric antibody is purified by Protein A affinity chromatography. As verified by binding test with N-NTD (Cat. No. NUN-C5143) and N-CTD (Cat. No. NUN-C5145) protein, this antibody can only bind to N-CTD (AA Ser 255 - Pro 364) . It can also bind multiple N protein variants with similar affinity as compared to the wild type N protein (Cat. No. NUN-C5227) .

Product Specifications

Background

Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68) . The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

Host

CHO

Origin Species

Mouse

Target

Nucleocapsid protein

Conjugation

Unconjugated

Tag

Human IgG1 | Human Kappa

Stability

● For long term storage, the product is stable for up to 3 years at -70°C from date of receipt; ● For short term storage, the product is stable for up to 12 months at 2-8°C from date of receipt. Please avoid repeated freeze-thaw cycles.

Purity

95%

Bioactivity

Immobilized Anti-SARS-CoV-2 Nucleocapsid Antibody, Human IgG1 (AM223) (Cat. No. NUN-CH15) at 1μg/mL (100μL/well) can bind SARS-CoV-2 Nucleocapsid protein, His Tag (B.1.1.529/Omicron) (Cat. No. NUN-C52Ht) with a linear range of 0.2-6 ng/mL (Routinely tested).

Format

Liquid

Buffer

PBS, pH7.4

Shipping Conditions

Dry ice

Storage Conditions

-70°C

Package Size

200ug*1

Product Datasheet

Https://www.acrobiosystems.com/static/main/products/pdf/ds/NUN-CH15.pdf

Species

Mouse

CAS Number

9007-83-4

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