Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS47)

Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS47) (NUN-S47L8) was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant SARS-CoV-2 Nucleocapsid protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Product Specifications

Background

Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68) . The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

CAS Number

9007-83-4

Host

HEK293

Origin Species

Mouse

Target

Nucleocapsid protein

Conjugation

Biotin-labeled

Tag

Mouse IgG1 | Mouse Kappa

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles.

Purity

0.95

Bioactivity

<strong>Detection SARS-CoV-2 Nucleocapsid Protein by Sandwich ELISA Assay.</strong><br> Immobilized Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS46) (Cat. No. NUN-S46) at 4 μg/mL (100 μL/well) can bind SARS-CoV-2 Nucleocapsid protein, His Tag (Cat. No. NUN-C5227). And then add Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS47) (Cat. No. NUN-S47L8) at 0.05 µg/mL. Detection was performed using high sensitivity HRP-conjugated streptavidin with sensitivity of 12.5 pg/mL (QC tested).