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FITC-Labeled Human IgG1 Kappa Isotype Control (FITC, mAb, carrier free)

Dinitrophenyl (DNP) is a hapten that is not normally expressed in the target tissue. Therefore, anti-DNP antibody has no relevant specificity to a target antigen, and can be used as isotype control antibody to differentiate non-specific background signal from specific antibody signal in various in vitro and in vivo studies. FITC-Labeled Human IgG1 Kappa Isotype Control (mAb) was purified from HEK293 cell culture. It is the FITC labeled form of Human IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M2) .

Product Specifications

Background

A hapten is a small molecule that can elicit an immune response only when conjugated with a large carrier such as a protein. Typical haptens include drugs, urushiol, quinone, steroids, etc. Peptides and non-protein antigens usually need conjugating to a carrier protein (such as BSA (bovine serum albumin) or KLH (keyhole limpet hemocyanin) to become good immunogens) . Additionally, haptens should be administered with an adjuvant to ensure a high quality immune response. It is important that the hapten design (preserving greatly the chemical structure and spatial conformation of target compound), selection of the appropriate carrier protein and the conjugation method are key conditions for the desired specificity anti-hapten antibodies. We design anti-hapten antibodies based on the HaptenDB information.

Host

HEK293

Origin Species

Human

Target

IgG control

Conjugation

FITC Labeled

Applications

This antibody is suitable for use as a non-targeting isotype control in various in vitro and in vivo studies. It can also be used as a negative control in various applications such as ELISA, Western blot, immunofluorescence, immunohistochemistry, immunoprecipitation, and flow cytometry. Each laboratory should determine an optimum working titer for use in its particular application.

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 12 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please protect from light and avoid repeated freeze-thaw cycles.

Purity

95%

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C, avoid light

Package Size

25ug*1

Host or Source

HEK293

Species

Human

Preservative

Trehalose

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