Biotinylated Human Insulin R / CD220 (28-944) Protein, His, Avitag™ (MALS & SPR verified)

Insulin receptor (INSR) is also known as CD antigen CD220, which can be cleaved into the following 2 chains: Insulin receptor subunit alpha and Insulin receptor subunit beta. INSR is a tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains carry the insulin-binding regions, while the beta chains carry the kinase domain. Forms a hybrid receptor with IGF1R, the hybrid is a tetramer consisting of 1 alpha chain and 1 beta chain of INSR and 1 alpha chain and 1 beta chain of IGF1R. In addition to binding insulin, the insulin receptor can bind insulin-like growth factors (IGFI and IGFII) . Isoform Short of INSR has a higher affinity for IGFII binding. When present in a hybrid receptor with IGF1R, INSR binds IGF1.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 108.2 kDa. The protein migrates as 115-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P06213-2

Expression Region

His 28 - Lys 944

Host

HEK293

Target

Insulin R

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.