Human Claudin-1 Protein, Flag, His Tag (Detergent)

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. [provided by RefSeq, Jul 2008]

This protein carries a flag tag at the N-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 25.8 kDa. The protein migrates as 8 kDa, 10 kDa, 20 kDa and 22-23 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) .

Immobilized Human Claudin-1 Protein, Flag,His Tag (Cat. No. CL1-H55D8) at 5 μg/mL (100 μL/well) on a Nickel Coated plate can bind Monoclonal Anti-Human Claudin-1 antibody, Human IgG1 with a linear range of 0.001-0.625 μg/mL (QC tested).