Biotinylated Human HLA-A*02:01&B2M&PSMA (MMNDQLMFL) Complex Protein (Monomer, MALS verified)

Prostate-specific membrane antigen (PSMA) is also known as Folate hydrolase 1 (FOLH1), Glutamate carboxypeptidase 2 (GCP2), N-acetylated-alpha-linked acidic dipeptidase I (NAALAD1), which belongs to the peptidase M28 family and M28B subfamily. FOLH1 / PSMA is a type II transmembrane zinc metallopeptidase that is most highly expressed in the nervous system, prostate, kidney, and small intestine. The Human HLA-A*0201 PSMA (MMNDQLMFL) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M and MMNDQLMFL peptide of the PSMA.

Product Specifications

Background

Specifications

Biotinylated Human HLA-A*02:01&B2M&PSMA (MMNDQLMFL) Complex Protein is produced by co-expression of HLA and B2M loaded with PSMA peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 36.3 kDa and 11.7 kDa. The protein migrates as 40-43 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

AAA59606.1 (HLA-A*02:01) & P61769 (B2M) & MMNDQLMFL

Expression Region

Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & MMNDQLMFL peptide

Host

HEK293

Target

HLA-A*0201 & B2M & PSMA (MMNDQLMFL)

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Biotinylated Human HLA-A*02:01&B2M&PSMA (MMNDQLMFL) Complex Protein (Cat. No. HLA-H82E6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).