Influenza A (A/Shanghai/02/2013 (H7N9) ) Hemagglutinin (HA) Protein, His Tag

Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Hemagglutinin also plays a major role in the determination of host range restriction and virulence. As a class I viral fusion protein, hemagglutinin is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 61.2 kDa. The protein migrates as 70-85 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

R4NN21_9INFA (339-340deletion)

Expression Region

Asp 19 - Gly 338 & Leu 341 - Asp 523

Host

HEK293

Target

Hemagglutinin/HA (Influenza Virus)

Conjugation

Unconjugated

Tag

C-10xHis

Source

Influenza

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Influenza A (A/Shanghai/02/2013(H7N9)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA9-V52H3) at 2 μg/mL (100 μL/well) can bind Rabbit anti-Influenza A virus(H7N9) Hemagglutinin polyclonal Antibody with a linear range of 0.005-0.078 μg/mL (QC tested).