Human HLA-A*03:01&B2M&KRAS (VVVGAGGVGK) Complex Protein (Monomer, MALS verified)
Human HLA-A*03:01&B2M&KRAS (VVVGAGGVGK) Complex Protein (HLS-H52H7) is expressed from human 293 cells (HEK293) . It contains AA Gly 25 - Thr 305 (HLA-A*03:01) & Ile 21 - Met 119 (B2M) & VVVGAGGVGK peptide (Accession # P04439 (HLA-A*03:01) & P61769 (B2M) & VVVGAGGVGK) .
Product Specifications
Background
The Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) oncogene plays a critical role in the initiation and maintenance of pancreatic tumors and its signaling network represents a major target for therapeutic intervention. The Human HLA-A*0301 KRAS (VVVGAGGVGK) complex protein is a complex of HLA-A*0301 of the MHC Class I, B2M, and VVVGAGGVGK peptide of the KRAS.
Specifications
Human HLA-A*03:01&B2M&KRAS (VVVGAGGVGK) Complex Protein is produced by co-expression of HLA and B2M loaded with KRAS peptide. This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 37.7 kDa and 15.7 kDa. The protein migrates as 42-45 kDa and 15 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
P04439 (HLA-A*03:01) & P61769 (B2M) & VVVGAGGVGK
Expression Region
Gly 25 - Thr 305 (HLA-A*03:01) & Ile 21 - Met 119 (B2M) & VVVGAGGVGK peptide
Host
HEK293
Target
HLA-A*0301 & B2M & KRAS (VVVGAGGVGK)
Conjugation
Unconjugated
Tag
C-10xHis
Source
Human
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
90%
Bioactivity
Immobilized Human HLA-A*03:01&B2M&KRAS (VVVGAGGVGK) Complex Protein (Cat. No. HLS-H52H7) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.05-1 ng/mL (QC tested).
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
37.7 kDa and 15.7 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
200ug*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/HLS-H52H7.pdf
Species
Human
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