Biotinylated Human HLA-A*03:01&B2M&KRASG12C (VVVGACGVGK) Complex Protein (Monomer, MALS verified)
Biotinylated Human HLA-A*03:01&B2M&KRASG12C (VVVGACGVGK) Complex Protein (HLC-H82E3) is expressed from human 293 cells (HEK293) . It contains AA Gly 25 - Thr 305 (HLA-A*03:01) & Ile 21 - Met 119 (B2M) & VVVGACGVGK peptide (Accession # P04439 (HLA-A*03:01) & P61769 (B2M) & VVVGACGVGK) .
Product Specifications
Background
The Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) oncogene plays a critical role in the initiation and maintenance of pancreatic tumors and its signaling network represents a major target for therapeutic intervention. The Human HLA-A*0301 KRASG12C (VVVGACGVGK) complex protein is a complex of HLA-A*0301 of the MHC Class I, B2M, and VVVGACGVGK peptide of the KRASG12C.
Specifications
Biotinylated Human HLA-A*03:01&B2M&KRASG12C (VVVGACGVGK) Complex Protein is produced by co-expression of HLA and B2M loaded with KRASG12C peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 35.9 kDa and 11.7 kDa. The protein migrates as 66 kDa, 55 kDa, 41-43 kDa, 34 kDa, 27 kDa and 11 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
P04439 (HLA-A*03:01) & P61769 (B2M) & VVVGACGVGK
Expression Region
Gly 25 - Thr 305 (HLA-A*03:01) & Ile 21 - Met 119 (B2M) & VVVGACGVGK peptide
Host
HEK293
Target
HLA-A*0301 & B2M & KRASG12C (VVVGACGVGK)
Conjugation
Biotin-labeled
Tag
C-10xHis & C-Avi
Source
Human
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
90%
Bioactivity
Immobilized Biotinylated Human HLA-A*03:01&B2M&KRASG12C (VVVGACGVGK) Complex Protein (Cat. No. HLC-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
35.9 kDa and 11.7 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
25ug*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/HLC-H82E3.pdf
Species
Human
Curated Selection
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