Unconjugated Human HLA-A*11:01&B2M&KRASG12V (VVVGAVGVGK) Complex Protein (Monomer, MALS verified)

The Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) oncogene plays a critical role in the initiation and maintenance of pancreatic tumors and its signaling network represents a major target for therapeutic intervention. The Human HLA-A*1101 KRASG12V (VVVGAVGVGK) complex protein is a complex of HLA-A*1101 of the MHC Class I, B2M, and VVVGAVGVGK peptide of the KRASG12V.

Unconjugated Human HLA-A*11:01&B2M&KRASG12V (VVVGAVGVGK) Complex Protein is produced by co-expression of HLA and B2M loaded with KRASG12V peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™), and it is not biotinylated. The protein has a calculated MW of 36.0 kDa and 11.7 kDa. The protein migrates as 40-42 kDa and 11 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Immobilized Unconjugated Human HLA-A*11:01&B2M&KRASG12V (VVVGAVGVGK) Complex Protein (Cat. No. HLV-H52E5) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-4 ng/mL (QC tested).