Fluorescent Human Claudin-6 / CLDN6 Full Length Protein, Flag, His, GFP Tag (Nanodisc)

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space.

Product Specifications

Background

Specifications

This protein carries a flag tag at the N-terminus, followed by a polyhistidine tag, and a GFP tag at the C-terminus. The protein has a calculated MW of 54.0 kDa. The protein migrates as 22 kDa, 25 kDa, 51-52 kDa and 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P56747

Expression Region

Ala 2 - Val 220

Host

HEK293

Target

Claudin-6

Conjugation

GFP Fusion

Tag

N-Flag & N-10xHis & C-GFP

Source

Human

Stability

The product MUST be stored at -70°C or lower upon receipt; -70°C for 3 months under sterile conditions.

Endotoxin

1.0 EU per μg

Purity

80%

Bioactivity

Immobilized Fluorescent Human Claudin-6 Protein, Flag,His,GFP Tag (Cat. No. CL6-HF2M9) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human Claudin 6 antibody, Human IgG1 with a linear range of 0.1-3 ng/mL (QC tested).