Biotinylated SARS-CoV-2 Spike RBD Protein, His, Avitag™ (XBB.1.16/Omicron) (MALS verified)

Biotinylated SARS-CoV-2 Spike RBD Protein, His, Avitag (XBB.1.16/Omicron) (SPD-C82Q4) is expressed from human 293 cells (HEK293) . It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478R, E484A, F486P, F490S, Q498R, N501Y, Y505H) ) . The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: XBB.1.16) .

Product Specifications

Background

It's been reported that coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 28.3 kDa. The protein migrates as 35-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

QHD43416.1 (G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478R, E484A, F486P, F490S, Q498R, N501Y, Y505H)

Expression Region

Arg 319 - Lys 537

Host

HEK293

Target

Spike RBD

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

SARS-CoV-2

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 5 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 Spike RBD Protein, His,Avitag (XBB.1.16/Omicron) (Cat. No. SPD-C82Q4) with a linear range of 1-16 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

28.3 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

Storage Conditions

-20°C

Package Size

200ug*1

Host or Source

HEK293

Species

SARS-CoV-2

Protein ID

QHD43416.1 (G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478R, E484A, F486P, F490S, Q498R, N501Y, Y505H)