Biotinylated Human HLA-A*02:01&B2M&KRASG12V (VVVGAVGVGK) Complex Protein (Monomer, MALS verified)

The Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) oncogene plays a critical role in the initiation and maintenance of pancreatic tumors and its signaling network represents a major target for therapeutic intervention. The Human HLA-A*0201 KRASG12V (VVVGAVGVGK) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and VVVGAVGVGK peptide of the KRASG12V.

Product Specifications

Background

Specifications

Biotinylated Human HLA-A*02:01&B2M&KRASG12V (VVVGAVGVGK) Complex Protein is produced by co-expression of HLA and B2M loaded with KRASG12V peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 36.3 kDa and 11.7 kDa. The protein migrates as 40-43 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & VVVGAVGVGK

Expression Region

Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & VVVGAVGVGK peptide

Host

HEK293

Target

HLA-A*0201 & B2M & KRASG12V (VVVGAVGVGK)

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Biotinylated Human HLA-A*02:01&B2M&KRASG12V (VVVGAVGVGK) Complex Protein (Cat. No. HLV-H82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

36.3 kDa and 11.7 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

Storage Conditions

-20°C

Package Size

25ug*1

Host or Source

HEK293

Species

Human

Protein ID

AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & VVVGAVGVGK

Preservative

Trehalose

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