Sus scrofa CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Tag Free (MALS verified)
Sus scrofa CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (CDD-S52W3) is expressed from human 293 cells (HEK293) . It contains AA Glu 23 - Leu 116 (CD3E) & Tyr 22 - Leu 104 (CD3D) (Accession # NP_999392.1 (CD3E) & NP_998940.1 (CD3D) ) .
Product Specifications
Background
T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon (CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
Specifications
Sus scrofa CD3E&CD3D Heterodimer Protein, His Tag&Tag Free is produced by co-expression of CD3E and CD3D, has a calculated MW of 16.2 kDa (CD3E) and 13.5 kDa (CD3D) . Subunit CD3E is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag and subunit CD3G contains no tag at the C-terminus. As a result of glycosylation, the protein migrates as 19-22 kDa under reducing (R) condition, and 35-38 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) .
Accession Number
NP_999392.1 (CD3E) & NP_998940.1 (CD3D)
Expression Region
Glu 23 - Leu 116 (CD3E) & Tyr 22 - Leu 104 (CD3D)
Host
HEK293
Target
CD3E & CD3D
Conjugation
Unconjugated
Tag
C-10xHis | Native
Source
Sus scrofa (Pig)
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
90%
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
16.2 kDa (CD3E) & 13.5 kDa (CD3D)
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
50ug*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/CDD-S52W3.pdf
Species
Sus scrofa (Pig)
Curated Selection
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