Sus scrofa CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Tag Free (MALS verified)

T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon (CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Product Specifications

Background

Specifications

Sus scrofa CD3E&CD3D Heterodimer Protein, His Tag&Tag Free is produced by co-expression of CD3E and CD3D, has a calculated MW of 16.2 kDa (CD3E) and 13.5 kDa (CD3D) . Subunit CD3E is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag and subunit CD3G contains no tag at the C-terminus. As a result of glycosylation, the protein migrates as 19-22 kDa under reducing (R) condition, and 35-38 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) .

Accession Number

NP_999392.1 (CD3E) & NP_998940.1 (CD3D)

Expression Region

Glu 23 - Leu 116 (CD3E) & Tyr 22 - Leu 104 (CD3D)

Host

HEK293

Target

CD3E & CD3D

Conjugation

Unconjugated

Tag

C-10xHis | Native

Source

Sus scrofa (Pig)

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

16.2 kDa (CD3E) & 13.5 kDa (CD3D)

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

Storage Conditions

-20°C

Package Size

50ug*1

Host or Source

HEK293

Species

Sus scrofa (Pig)

Protein ID

NP_999392.1 (CD3E) & NP_998940.1 (CD3D)

Preservative

Trehalose

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