Unconjugated Human HLA-A*02:01&B2M Monomer Protein (Peptide free, MALS verified)

Unconjugated Human HLA-A*02:01&B2M Monomer Protein (HLM-H52H5) is expressed from human 293 cells (HEK293) . It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) ) .

Product Specifications

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™), and it is not biotinylated. The protein has a calculated MW of 36.3 kDa and 11.7 kDa. The protein migrates as 38-42 kDa and 10-11 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M)

Expression Region

Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M)

Host

HEK293

Target

HLA-A*0201 & B2M

Conjugation

Unconjugated

Tag

C-10xHis & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Unconjugated Human HLA-A*02:01&B2M Monomer Protein (Cat. No. HLM-H52H5) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.5-4 ng/mL (QC tested).