Biotinylated Human HLA-A*02:01&B2M&MAGE-A3 (KVAELVHFL) Complex Protein (Monomer, MALS verified)
Biotinylated Human HLA-A*02:01&B2M&MAGE-A3 (KVAELVHFL) Complex Protein (HLM-H82E3) is expressed from human 293 cells (HEK293) . It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & KVAELVHFL peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & KVAELVHFL) .
Product Specifications
Background
The MAGE-A3 antigen is a cancer-testis antigen and is expressed intracellularly in various solid tumor tissues, MAGE-A3 112-120 peptide (KVAELVHFL) is a cytotoxic T lymphocyte (CTL) epitope presented by HLA-A*0201. The Human HLA-A*0201 MAGE-A3 (KVAELVHFL) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and KVAELVHFL peptide of the MAGE-A3.
Specifications
Biotinylated Human HLA-A*02:01&B2M&MAGE-A3 (KVAELVHFL) Complex Protein is produced by co-expression of HLA and B2M loaded with MAGE-A3 peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein migrates as 38-44 kDa and 12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & KVAELVHFL
Expression Region
Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & KVAELVHFL peptide
Host
HEK293
Target
HLA-A*0201 | B2M | MAGE-A3
Conjugation
Biotin-labeled
Tag
C-10xHis & C-Avi
Source
Human
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
90%
Bioactivity
Immobilized Biotinylated Human HLA-A*02:01&B2M&MAGE-A3 (KVAELVHFL) Complex Protein (Cat. No. HLM-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-1 ng/mL (QC tested).
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
36.3 kDa and 11.7 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
25ug*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/HLM-H82E3.pdf
Species
Human
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