Human ALPI Protein, His Tag (active enzyme, MALS verified)
Human ALPI Protein, His Tag (ALI-H52H3) is expressed from human 293 cells (HEK293) . It contains AA Val 20 - Asp 503 (Accession # P09923-1) .
Product Specifications
Background
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific) . The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is a component of the gut mucosal defense system and is thought to function in the detoxification of lipopolysaccharide, and in the prevention of bacterial translocation in the gut.
Specifications
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 54.3 kDa. The protein migrates as 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
P09923-1
Expression Region
Val 20 - Asp 503
Host
HEK293
Target
ALPI
Conjugation
Unconjugated
Tag
C-10xHis
Source
Human
Stability
● The product MUST be stored at -70°C or lower upon receipt; ● -70°C for 3 months under sterile conditions.
Endotoxin
1.0 EU per μg
Purity
95%
Bioactivity
Immobilized Human ALPI Protein, His Tag (Cat. No. ALI-H52H3) at 1 μg/mL (100 μL/well) can bind various dilution ratio of Anti-ALPI Polyclonal Antibody (QC tested).
Format
Liquid
Buffer
20 mM Tris, 150 mM NaCl, pH7.5
Additives
Trehalose
Molecular Weight
54.3 kDa
Shipping Conditions
Dry ice
Storage Conditions
-70°C
Package Size
100ug*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/ALI-H52H3.pdf
Species
Human
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