Biotinylated Human HLA-A*02:01&B2M&p53 (HMTEVVRHC) Complex Protein (Monomer, MALS verified)

TP53 has been recognized as a tumor suppressor. 50% of cancers carry a TP53 mutation while many others affect other pathway components. High-copy numbers of WT p53 peptide-MHC class I complexes were detected on tumor cells as compared to low copies on normal cells. The Human HLA-A*0201 p53 (HMTEVVRHC) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and HMTEVVRHC peptide of the p53.

Product Specifications

Background

Specifications

Biotinylated Human HLA-A*02:01&B2M&p53 (HMTEVVRHC) Complex Protein is produced by co-expression of HLA and B2M loaded with p53 peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 49.3 kDa. The protein migrates as 58-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P61769-1 (B2M) & AAA59606.1 (HLA-A*02:01) & HMTEVVRHC

Expression Region

Ile 21 - Met 119 (B2M) & Gly 25 - Ile 308 (HLA-A*02:01) & HMTEVVRHC peptide

Host

HEK293

Target

HLA-A*0201 & B2M & p53 (HMTEVVRHC)

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Biotinylated Human HLA-A*02:01&B2M&p53 (HMTEVVRHC) Complex Protein (Cat. No. HLP-H82Ef) at 1 μg/mL (100 μL/well) on a Nickel Coated plate can bind Anti-HLA-A2&p53R175H Antibody, Human IgG1 with a linear range of 0.2-4 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

49.3 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

Storage Conditions

-20°C

Package Size

200ug*1

Host or Source

HEK293

Species

Human

Protein ID

P61769-1 (B2M) & AAA59606.1 (HLA-A*02:01) & HMTEVVRHC

Preservative

Trehalose

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

NB100-426AF647 0.1 mL

Rabbit Polyclonal SCC112 Antibody [Alexa Fluor 647]

Ask

View Details

MBS1284308-01 0.02 mg (E-Coli)

Recombinant Aeropyrum pernix Putative ribonuclease VapC3 (vapC3)

Ask

View Details

MBS1284308-02 0.1 mg (E-Coli)

Recombinant Aeropyrum pernix Putative ribonuclease VapC3 (vapC3)

Ask

View Details

MBS1284308-03 0.02 mg (Yeast)

Recombinant Aeropyrum pernix Putative ribonuclease VapC3 (vapC3)

Ask

View Details

MBS1284308-04 0.1 mg (Yeast)

Recombinant Aeropyrum pernix Putative ribonuclease VapC3 (vapC3)

Ask

View Details

MBS1284308-05 0.02 mg (Baculovirus)

Recombinant Aeropyrum pernix Putative ribonuclease VapC3 (vapC3)

Ask

View Details