SARS coronavirus CUHK-W1 Spike NTD Protein, His Tag (MALS verified)

It's been reported that coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 33.2 kDa. The protein migrates as 47-64 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

AAP13567.1

Expression Region

Asp 17 - Lys 291

Host

HEK293

Target

Spike NTD

Conjugation

Unconjugated

Tag

C-10xHis

Source

SARS coronavirus CUHK-W1

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized SARS coronavirus CUHK-W1 Spike NTD Protein, His Tag (Cat. No. SPD-S52H9) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike NTD Antibody, Chimeric mAb, Human IgG1 (AM121) (Cat. No. SPD-M121) with a linear range of 0.5-8 ng/mL (QC tested).