Biotinylated Human IL-3 R alpha / CD123 Protein, Avitag™ (MALS verified)

Interleukin 3 receptor alpha (low affinity) (IL3RA), also known as CD123 (Cluster of Differentiation 123) is a 70-kD glycoprotein member of the hematopoietin receptor superfamily. This protein associates with a beta subunit common to the receptors for IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF) to form a high-affinity receptor for IL-3. The interleukin-3 receptor α chain (CD123) has been identified as a potential immunotherapeutic target because it is overexpressed in AML compared with normal hematopoietic stem cells.

Product Specifications

Background

Specifications

This protein carries an Avi tag (Avitag™) at the C-terminus. The protein has a calculated MW of 35.3 kDa. The protein migrates as 53-63 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P26951-1

Expression Region

Thr 19 - Arg 305

Host

HEK293

Target

IL-3 R alpha

Conjugation

Biotin-labeled

Tag

C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Biotinylated Human IL-3 R alpha Protein, Avitag (Cat. No. ILA-H8214) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-IL-3 R alpha Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).