HIV (HIV1-HXB2) Capsid protein p24, His Tag (MALS verified)

The Gag protein from retroviruses, also known as p24, forms the inner protein layer of the nucleocapsid. It is composed of two domains, the N-terminal domain (NTD), which contributes to viral core formation, and the C-terminal domain (CTD), which is required for capsid dimerisation, Gag oligomerization and viral formation. This protein performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 27.5 kDa. The protein migrates as 26-27 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P04591

Expression Region

Pro 133 - Leu 363

Host

HEK293

Target

Capsid protein p24/Gag p24 (HIV)

Conjugation

Unconjugated

Tag

C-10xHis

Source

HIV (HV1-HXB2)

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized HIV (HIV1-HXB2) Capsid protein p24, His Tag (Cat. No. CP4-H52H3) at 1 μg/mL (100 μL/well) can bind HIV1 p24 Monoclonal Antibody ,Mouse IgG2a with a linear range of 0.1-2 ng/mL (QC tested).