FITC-Labeled Rabies virus (strain CVS-11) Glycoprotein G, His Tag

Rabies virus (RABV), scientific name Rabies lyssavirus, is a deadly neurotropic virus that causes rabies in humans and animals. Rabies virus has an extremely wide host range and its transmission most often occur through the saliva of animals. Without intervention prior to disease progression, rabies has the highest case fatality of any infectious disease. RABV contains a single-stranded negative-sense RNA genome that encodes five structural proteins: nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G), and RNA-dependent RNA polymerase (L) . Among these viral proteins, the RABV glycoprotein (RABV-G) is a pivotal player mediating virus entry and the major target of neutralizing antibodies, thus a key factor for vaccine and drug design.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 53.6 kDa. The protein migrates as 60-62 kDa and >116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

GQ918139.1

Host

Insect Cells

Target

Glycoprotein/G Protein (RABV)

Conjugation

FITC Labeled

Tag

C-10xHis

Source

Rabies virus

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized FITC-Labeled Rabies virus (strain CVS-11) Glycoprotein G, His Tag (Cat. No. GLN-RF5E3) at 1 μg/mL (100 μL/well) can bind Human GFR alpha-2, Fc Tag with a linear range of 0.1-3 ng/mL (Routinely tested).